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RobotOligo is a flexible and intuitive fully automatic oligonucleotide synthesizer that can be used for 1μmol-12mmol rapid synthesis of nucleic
acid samples, suitable for clinical research, pharmaceutical development and synthesis of molecular diagnostic probes.
RobotOligo family members:
RobotOligo25, 1~100umol
RobotOligo100, 50μmol~9mmol
RobotOligo150, 150μmol~12 mmol
RobotOligo600, 1mmol-50mmol
RobotOligo metering pump drive system with high precision, compatible with reagent nucleic acid synthesis, flow-through type solid phase
column synthesis technology, can significantly reduce the reagent consumption, and ensure accurate control of the reaction speed and
contact time, synthesis is more efficient, easy to scale up, cost-effective and high quality.
RobotOligo can use up to 14 monomers simultaneously, continuously and automatically performing the synthesis process on one or three or
eight columns.
RobotOligo System workstation has a powerful data management System with complete and reliable nucleic acid synthesis method
development platform.
RobotOligo Application
Case One: DNA 17mer Synthesis
Purpose:
Synthesize a strand of oligonucleotide DNA, Sequence: CTACGCCACGAGCTCCA, Synthesis amount 0.24mmol
Experiments:
1) Oligo Synthesizer: GoodScience RobotOligo 100
2) Column: 6.3ml
3) Carrier: HYS 350
4) Deprotection reagent: coupling reagent, oxidation reagent, blocking reagent, anhydrous acetonitrile, ammonia
5) Monomer：A、C、G、T
6) 0.3bar Ammonia gas
7) Mass Spectrometer: WATERS – THERMO
8) HPLC: WATERS
RobotOligo Application
Case Two: DNA 63mer Oligonucleotide fragment synthesis
Purpose:
Synthesize a single strand of ssDNA, 63mer (not listed as sequence is confidential), Synthesis amount 174μmol, Purity > 95% after purification.
Experiments:
1) Oligo Synthesizer: GoodScience RobotOligo 100
2) Column: 6.3ml
3) Carrier：Nitto H09002
4) Reagents: deprotectant, activator, oxidant, thiolide, CAPA, CAPB, anhydrous acetonitrile, 20% DEA, ammonia.
5) Monomer：A、C、G、T、U
6) Protein Purification System: GoodScience Autopure 100
7) Mass Spectrometer：ThermoFisher LTQ
8) HPLC: GoodScience LC-3000
Results Analysis:
Purity of crude product is 46.3%, and purity reached 97.8% after one-step reverse-phase chromatography purification. Finally, the molecular weight of
the target product is 19344, which is matching with the theoretical molecular weight of the target product (19342.64).
RobotOligo Application
Case Three: RNA 21mer Synthesis
Purpose:
Synthesize a single strand of RNA, 21mer (not listed as sequence is confidential), Synthesis amount 245μmol.
Experiments:
Oligo Synthesizer: GoodScience RobotOligo 100
Results Analysis:
Purity of crude product is 84.84%, and purity reached 94.37% after purification. Molecular weight measured by mass spectrometry is 7097.7, which is
matching with theoretical value (7096.8).
RobotOligo Application
Case Four: RNA 76mer Synthesis
Purpose:
Synthesize a single strand of RNA, 76mer (not listed as sequence is confidential), Synthesis amount 70μmol.
Experiments:
Oligo Synthesizer: GoodScience RobotOligo 100
Results Analysis:
Purity of crude product is 69.61%, and purity reached 93.93% after purification. Molecular weight measured by mass spectrometry is 25133, which is
matching with theoretical value (25131.2).
Configuration Parameter